Coding

Part:BBa_K4011001:Design

Designed by: Zixiang Zhou   Group: iGEM21_LINKS_China   (2021-10-21)


CBM2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 52
    Illegal AgeI site found at 96
    Illegal AgeI site found at 268
    Illegal AgeI site found at 334
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

1. All codons were optimized for S. cerevisiae or E. coli based on S. cerevisiae or E. coli codon bias

2. Flexible linker GSGGGS used between first CBM2 and NT2RepCT, flexible linker GGGGS used between NT2RepCT and second CBM2.


Source

CBM2 is derived from Cellulomonas fimi.

References